A2A (Adenosine Receptor A2A)
SIGMA/SAE0103 - recombinant, expressed in Sf9 cells
Product Type: Chemical
SDS-PAGE Cat. No. SAE0103: Western blotting. Purified A2A was migrated on a 4-15% Tris-glycine SDS-PAGE, transferred to pvdf membrane and immunodetected with a monoclonal anti-ICL3-A2A (7F6-G5-A2, SCBT). Black arrows indicate the target.
SDS-PAGE Cat. No. SAE0103: Stain-Free detection. Purified A2A was migrated on a 4-15% Tris-glycine SDS-PAGE and the total proteins were Stain-Free detected. Black arrows indicate the target. Upper arrows indicate full-length A2A. Lower arrow indicates shorter A2A resulting from partial cleavage of C-term end.
Radio-binding Assay Cat. No. SAE0103: Activity measured by radio-binding assay. Binding of [3H]ZM241385 was measured on purified A2A. A KD of 6 nM was determined for ZM241385.
| assay | ≥90% (SDS-PAGE) |
| concentration | 1.72 mg/mL |
| description | N-Terminal contains a strep tag II and 10X Histidine tag followed by a TEV protease cleavage site. |
| form | aqueous solution |
| mol wt | 47.7 kDa |
| NCBI accession no. | NC_000022.11   |
| recombinant | expressed in Sf9 cells |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | P29274 |
| Biochem/physiol Actions: | A2A is a class A GPCR involved in regulating myocardial blood flow and hypertension. |
| Preparation Note: | 50mM Hepes pH 7.4, 200mM NaCl, 0.05%/0.006% DDM/CHS |
| Sequence: | MWSHPQFEKHHHHHHHHENLYFQGP |
| Storage and Stability: | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot into single use aliquots. Store remaining undiluted protein in aliquots at -70°C. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352202 |