Chitinase from Streptomyces griseus
SIGMA/SAE0158 - chromatographically purified, lyophilized powder, free of DNA contaminants, suitable for Microbiome research
Synonym: Chitinase from Streptomyces griseus; (1→4)-2-acetamido-2-deoxy-beta-D-glucan glycanohydrolase); Chitodextrinase; Hydrolitic enzyme; Lytic Enzyme; Poly(β-(1→4)-[2-acetamido-2-deoxy-D-glucoside]) glycanohydrolase; Poly(1,4-β-[2-acetamido-2-deoxy-D-glucoside]) glycanohydrolase; beta-
Product Type: Chemical
PCR amplification of 16S rDNA using universal primer set Chitinase from Streptomyces griseus (SAE0158). PCR amplification was conducted using 16S universal primers to amplify a 1500 bp amplicon. Lanes M: 1 kb ladder (D3937) 1. Positive control containing only genomic DNA from Proteus mirabilis 2. Negative control with water only 3. Heat inactivated Chitinase 4. Chitinase spiked with genomic DNA Heat inactivated Chitinase is shown to be free of DNA contaminates by lack of amplified 16S amplicon in reaction containing only sample (Lane 3) compared to successful amplification of 16S amplicon in reaction spike with genomic DNA (Lane 4)
| feature | DNA free |
| Quality Level | 300  |
| shipped in | wet ice |
| specific activity | >200 USP units/mg solid |
| storage temp. | −20°C |
| Application: | • Agriculture fields: control pathogens. • Human health care: Asthma. • Pharma: preparation of chitooligosaccharides and N-acetyl D glucosamine, • Preparation of single-cell protein • Isolation of protoplasts from fungi and yeast • Control of pathogenic fungi • Treatment of chitinous waste, mosquito control and morphogenesis The study of microbial communities has recently been revolutionized by the widespread adoption of culture- independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminants are essential. Purified Chitinase SAE0158 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA, using 35 cycles of PCR amplification of 16S and 18S rDNA, using universal primer sets. |
| Features and Benefits: | This Chitinase is free of DNA contaminates, suitable for microbiome research. Cell walls digestion is performed safely by chitin degradation in fungi and other organisms. |
| General description: | Chitinase is an extracellular complex of enzymes that degrade chitin. Chitin is a cell wall component of Fungi and exoskeletal essentials of different organisms which reshape their own chitin or digest/dissolve the chitin of other organisms (insects, fungi, yeast, and algae, and in the internal structures of other vertebrates). Chitinases have been detected in many microorganisms and in plants. In fungi, chitinases assist in morphogenesis, to break down the inherent chitin content of fungal cell walls. Plant chitinases help in resistance to fungal attack and counteracting fungal growth, by targeting those same fungal cell walls. In bacteria, bacterial chitinases assist in utilizing chitin as a carbon source and as an energy source. Streptomyces griseus produces multiple chitinases of different molecular masses after growth induction with chitin as the carbon source. The enzymatic hydrolysis of chitin to N-acetyl-D-glucosamine involves two consecutive enzyme reactions: • The first reaction, chitodextrinase-chitinase • The second activity is N-acetyl-glucosaminidasec |
| Unit Definition: | One unit will liberate 1.0 mg of N-acetyl-D-glucosamine from chitin per hour at pH 6.0 at 25 °C in a 2-hour assay. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| WGK Germany | WGK 3 |
| activity | specific activity: >200 USP units/mg solid |
| Storage Temp. | −20°C |