3CL, Mpro protease fluorogenic substrate peptide
SIGMA/SAE0180 - AFC-Peptide substrate, lyophilized
Product Type: Chemical
| assay | ≥95% (HPLC) |
| form | solid |
| Quality Level | 200  |
| shipped in | ambient |
| storage temp. | −20°C |
| General description: | This fluorogenic peptide is a substrate for the Main protease from SARS-Cov2, 3CL protease (Mpro). This substrate allows measurement of the activity of 3CL protease using a standard plate reader or spectrophotometer. Proteolytic cleavage by 3CL protease will release the fluorescent AFC group from the peptide (sequence TSAVLQ). After release, an increase in fluoresence is obtained (Excitation = 400 nm, Emission = 505 nm). Peptide: Thr-Ser-Ala-Val-Leu-Gln-A Lyophilized from 0.1% TFA in H2O |
| Purity | ≥95% (HPLC) |
| Storage Temp. | −20°C |