Parathyroid Hormone (1-84) Human
SIGMA/SAE0192 - Recombinant expressed in E. coli
Synonym: PTH, Parathormone
Product Type: Chemical
Activity of Human Parathyroid Hormone (1-84) Cat. No. SAE0192 is demonstrated by its ability to stimulate increase of cellular cAMP concentration in bone osteosarcoma SAOS -2 cells. The cells were exposed to increasing concentrations of PTH for 5 min at 37℃. Cellular cAMP concentration was measured using cAMP Enzyme Immunoassay kit (Cat. No. CA201).
| assay | ≥95% (SDS-PAGE) |
| Quality Level | 100  |
| recombinant | expressed in E. coli |
| shipped in | ambient |
| storage temp. | −20°C |
| Biochem/physiol Actions: | PTH acts to increase the concentration of calcium in the blood by acting upon parathyroid hormone receptor in three parts of the body: In the bones- It enhances the release of calcium from the large reservoir contained in the bones. Bone resorption is the normal destruction of bone by osteoclasts, which are indirectly stimulated by PTH. Recombinant Human full length PTH 1-84 has potential as an anti-osteoporotic agent, due to its properties as a bone formation stimulant, it increases bone turnover, stimulating osteoblasts and reducing both vertebral and non-vertebral fractures. |
| Disclaimer: | This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices. |
| General description: | Parathyroid hormone (PTH), or parathormone, is secreted by the parathyroid glands as a polypeptide containing 84 amino acids. It acts to increase the concentration of calcium in the blood, whereas calcitonin (a hormone produced by the parafollicular cells of the thyroid gland) acts to decrease calcium concentration. Parathyroid Hormone 1-84 (full length) Human Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 84 amino acids (MW of ∼9.4kDa) with the following amino acid sequence: SVSEIQLMHN LGKHLNSMER VEWLRKKLQD VHNFVALGAP LAPRDAGSQR PRKKEDNVLV ESHEKSLGEA DKADVNVLTK AKSQ |
| Physical form: | The product is supplied as lyophilized powder, from a 0.2μm filtered concentrated solution in PBS pH7.4. |
| Storage and Stability: | It is recommended to reconstitute the lyophilized PTH in sterile purified water, at not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. Upon reconstitution PTH should be stored at 2-8 °C up to 7 days and for future use at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze-thaw cycles |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |