SB-MDR1-Hi5-PREDEASY-ATPase kit
SIGMA/SBPE01 - membrane based kit for Human MDR1 ATPase Transport Assays
Synonym: ABCB1; MDR1; MDR1 human membrane based kit for Human MDR1 ATPase Transport Assays; Multidrug resistance-
Product Type: Chemical
| recombinant | expressed in insect cells |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | P08183   |
| Application: | SOLVO′s PREDEASY™ ATPase Assay Kit is a unique product for measuring the ATPase-dependent drug - ABC membrane transporter interaction in a convenient, "ready-to-assay" package. The ATPase assay is an in vitro membrane assay designed to indicate the nature of the interaction between the compound and the transporter. By measuring ATPase activity, both activation and inhibition of transporters can be investigated using membranes from baculovirus-infected insect cells or mammalian cell membranes containing high levels of human or rodent wild-type transporters. ABC transporters mediate the transport of substrates against a concentration gradient using energy derived from ATP hydrolysis, which is proportional to the transporter activity and could easily be detected with a colorimetric method. PREDIVEZTM PREDEASY™ Kits contain all reagents (chemicals) and solutions necessary to conduct ATPase activation and inhibition assays. You only have to add water. Each kit comes with a CD that includes the assay protocol, data sheet, SDS, electronic data processing, evaluation templates and the reference data. The recommended layout allows activation and inhibition measurements in duplicates for 8 concentrations of the test article on one 96-well plate with all of the relevant controls. PREDEASY™ ATPase Assay Kits are available for 3 and 10 plates for the transporters recommended by the FDA and the EMA. |
| General description: | SOLVO Biotechnology offers ready-to-use assay kits for efflux and uptake transporters. SOLVO′s PREDEASY™ ATPase Assay Kit provides everything necessary to conduct ATPase activation and inhibition assays to better understand the nature of the interaction between a compound and the transporter. By measuring ATPase activity, both activation and inhibition of transporters can be investigated. |
| Legal Information: | 6538 is a trademark of American Type Culture Collection |
| Legal Information: | Distributed for SOLVO Biotechnology, Inc. |
| Legal Information: | PREDEASY is a trademark of Solvo Biotechnology |
| Physical form: | Supplied as frozen membrane vesicles, containing 5 mg/ml membrane protein, labeled with volume, catalog number (transporter), date of production, protocols and necessary reagents sufficient for the analysis of 3 or 10, 96-well plates. |
| Symbol |     GHS02,GHS05,GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H226 - H290 - H314 - H335 - H350 - H412 |
| Precautionary statements | P210 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| Hazard Codes | T |
| Risk Statements | 45-21/22-34-37-52/53 |
| Safety Statements | 53-26-36/37/39-45-61 |
| RIDADR | UN 3316 9 |
| WGK Germany | WGK 3 |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |