Actinomycin D, Ready Made Solution
SIGMA/SBR00013 - from Streptomyces sp., 2 mg/mL in DMSO
Synonym: Actinomycin D solution from Streptomyces sp.; Actinomycin IV; Dactinomycin
CAS Number: 50-76-0
Empirical Formula (Hill Notation): C62H86N12O16
Molecular Weight: 1255.42
Linear Formula: C62H86N12O16
Product Type: Chemical
antibiotic activity spectrum | neoplastics |
biological source | Streptomyces sp. |
color | dark orange |
concentration | 2 mg/mL in DMSO |
description | Ready Made Solution |
form | liquid |
InChI | 1S/C62H86N12O16/c1-27(2)4 |
InChI key | RJURFGZVJUQBHK-UHFFFAOYSA |
mode of action | DNA synthesis | interferes |
Quality Level | 200 ![]() |
storage condition | protect from light |
storage temp. | −20°C |
Biochem/physiol Actions: | Actinomycin D is an antineoplastic antibiotic that inhibits cell proliferation. It is a cytotoxic inducer of apoptosis against tumor cells. The compound inhibits the proliferation of cells in a nonspecific way by forming a stable complex with double-stranded DNA (via deoxyguanosine residues), thus inhibiting DNA-primed RNA synthesis. It also causes single strand breaks in DNA. Actinomycin D has been shown to be an inhibitor of the minus-strand transfer step in reverse transcription, and is therefore used in studies and suppression of HIV-replication. It has also been shown to suppress programmed cell death of PC12 cells induced by etoposide, an inhibitor of topoisomerase II. In cell culture, actinomycin D is used as a selection agent. Actinomycin D binding to yeast in ribosomal RNA has been studied. The sensitivity of various strains of E. coli to actinomycin D and the mechanism of binding has been studied. Actinomycin D is also used in molecular biology as a DNA intercalator which blocks the progression of RNA polymerases. |
Caution: | Actinomycin D solutions are very sensitive to light. |
Packaging: | 1ML |
Preparation Note: | The 2 mg/mL solution may be further diluted in an appropriate buffer prior to use. |
Symbol | ![]() |
Signal word | Danger |
Hazard statements | H350 |
Precautionary statements | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
WGK Germany | WGK 3 |
Flash Point(F) | 188.6 °F - closed cup |
Flash Point(C) | 87 °C - closed cup |
Storage Temp. | −20°C |
UNSPSC | 12352200 |