1-Cyano-4-dimethylaminopyridinium tetrafluoroborate Ready Made Solution
SIGMA/SBR00023 - organic cyanylating reagent, 100 mg/mL in acetonitrile
Synonym: 4-
CAS Number: 59016-56-7
Empirical Formula (Hill Notation): C8H10BF4N3
Molecular Weight: 234.99
Linear Formula: C8H10BF4N3
Product Type: Chemical
| assay | ≥97% |
| biological source | synthetic |
| concentration | 100 mg/mL in acetonitrile |
| form | liquid |
| shipped in | dry ice |
| storage temp. | −20°C |
| Application: | Polysaccharide conjugation: CDAP Ready Made Solution (100mg/mL in acetonitrile) should be added in a ratio of 1mg CDAP to 1mg polysaccharide while vortexing (Example: per 1mg polysaccharide add 10μL of CDAP Ready Made Solution). Add 0.2M triethylamine, 10μL per 1mg polysaccharide to raise the pH of the reaction. |
| Biochem/physiol Actions: | CDAP is a cyanylation reagent for protein sulfhydryl groups which is used to prepare protein-polysaccharide conjugates. CDAP is also an activating agent for polysaccharide resins and is used for the conjugation of lipopolysaccharides while retaining their endotoxic activity. CDAP is considered to be a less toxic reagent as compared to cyanogen bromide (CNBr) (a known polysaccharides activator). In addition, CDAP is easier to use as it can be employed at a lower pH and has fewer side reactions. It is known that CDAP polysaccharide activation efficiency is optimal at pH 9-10. It was also reported that direct conjugation of protein to CDAP-activated polysaccharides can be performed under mildly alkaline conditions (pH 7-9). It has also been reported that proteins could also be conjugated to CDAP-activated polysaccharides at pH 5. |
| Symbol |    GHS02,GHS05,GHS07 |
| Signal word | Danger |
| Hazard statements | H225 - H302 + H312 + H332 - H314 |
| Precautionary statements | P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| WGK Germany | WGK 3 |
| Flash Point(F) | 35.6 °F |
| Flash Point(C) | 2 °C |
| Purity | ≥97% |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |