Rhodamine B labeled Polymyxin B Ready Made Solution
SIGMA/SBR00036 - For fluorescent microbial imaging, 1.1 mg/mL in water
Synonym: SRB-PMB
Product Type: Chemical
concentration | 1.1 mg/mL in water |
Quality Level | 100 ![]() |
storage temp. | −20°C |
Analysis Note: | • Rhodamine B labeled polymyxin B Ready Made Solution is light sensitive. • It is recommended to avoid freeze-thaw cycles of Rhodamine B labeled polymyxin B Ready-Made Solution • Rhodamine B labeled polymyxin B Ready Made Solution (1.1mg/mL) can be diluted 1:100-200 in PBSX1 (Product# D8537) to achieve 0.55-1.1μg/mL final concentration for staining. The above concentration of Rhodamine B labeled polymyxin B was used for Escherichia coli staining (see image) • Fluorescence Microscopy application: Rhodamine B labeled polymyxin B Ready Made Solution excitation (Ex) wavelength is 550-560nm resulting in emission (Em) range of 580-610nm (λmax=590-600nm) |
Application: | Fluorescent antibiotics can be used for many applications including:• Antimicrobial resistance research. • Bacterial visualization and imaging. • Parent antibiotic mode of action research and new antibiotic discovery. • Toxicity studies. • Research of bacterial infections and tracking its uptake in vivo. Additional possible applications for Rhodamine B labeled Polymyxin B:• Evaluate LPS binding by FRET (fluorescence resonance energy transfer) studies to form a specific complex between fluorescein (FITC)-tagged LPS and Polymyxin B, indicating direct binding of the antibiotic to the LPS • Can be applied as a fluorescent probe to study polymyxin mode of action and its pharmacokinetics • to assess the mitochondrial function of polymyxin B in a kidney cell line (LLC-PK1), suggesting that it changes the mitochondrial membrane polarization. |
General description: | Rhodamine B labeled polymyxin B (SRB-PMB) is a fluorescent derivative of Polymyxin B. Rhodamine B polymyxin B is an additional fluorescent derivative of Polymyxin B, along with Dansyl labeled polymyxin B (Product # SBR00029). Both products′ mode of action is in accordance with Polymyxin B activity. Fluorescent antibiotics are obtained by synthetic conjugation of an antibiotic to a fluorophore. |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | −20°C |