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Hygromycin B, Min. 95% (HPLC ELSD) Ready Made Solution

SIGMA/SBR00039 - 50 mg/mL in water (from Streptomyces hygroscopicus)

Synonym: Hygromycin B solution from Streptomyces hygroscopicus

CAS Number: 31282-04-9
Empirical Formula (Hill Notation): C20H37N3O13
Molecular Weight: 527.52
MDL Number: MFCD06795479
Linear Formula: C20H37N3O13
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-SBR00039-10ML 10 mL
 $221.00
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antibiotic activity spectrum fungi
  Gram-negative bacteria
  Gram-positive bacteria
  viruses
biological source Streptomyces hygroscopicus
concentration 50 mg/mL in water (from Streptomyces hygroscopicus)
InChI 1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1
InChI key GRRNUXAQVGOGFE-XKIAHZFYSA-N
mode of action protein synthesis | interferes
Quality Level 200 
SMILES string CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3OC4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O
storage temp. 2-8°C
Application: Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/mL, specifically at 100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes.
Biochem/physiol Actions: Mode of Action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.
Biochem/physiol Actions: Mode of action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.

Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.
Features and Benefits: Ready made solution for ease of use
General description: Chemical structure: Aminoglycoside
Storage Temp. 2-8°C

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