Rhodamine B labeled Ramoplanin
SIGMA/SBR00047 - For fluorescent microbial imaging
Empirical Formula (Hill Notation): C146H184ClN23O46S2
Molecular Weight: 3096.73
Linear Formula: C146H184ClN23O46S2
Product Type: Chemical
Quality Level | 100 ![]() |
specific activity | Antibiotic (bacterial staining) |
storage temp. | −20°C |
Analysis Note: | • Fluorescent microscopy application: Rhodamine B labeled Ramoplanin has excitation/emission wavelength range at 550-560 nm/590-600 nm. • It is recommended to dissolve the Rhodamine B labeled Ramoplanin in DMSO to a concentration of 2 mg/mL. • The recommended working concentration in fluorescent microscopy imaging application is 10μM. The mentioned concentration was used for Bacillus subtills staining (see image). • Aliquots of the DMSO solution can be stored at −20 °C, protected from light for at least one month. |
Application: | Fluorescent antibiotics can be used for many applications including: • Antimicrobial resistance research • Bacterial visualization and imaging • Parent antibiotic mode of action research and new antibiotic discovery • Toxicity studies • Diagnosis of bacterial infections and tracking their uptake in vivo |
Biochem/physiol Actions: | Ramoplanin blocks the bacterial cell wall biosynthesis by specifically binding to the first sugar of lipid II, N-Acetylmuramic acid, thus interfering with the peptidoglycan production. Its mode of action is different from the d-Ala-d-Ala that are targeted by vancomycin and shows no cross-resistance with other glycopeptides. Ramoplanin is a potent antibiotic with an MIC value of =1 μg /ml and broad-spectrum activity against Gram-positive pathogens both in vitro and in vivo, including Enterococci, Staphylococci, Bacilli, Streptococci, Listeria monocytogenes, and Gram-positive anaerobes such as Clostridrium difficile. However, Rhodamine B labeled Ramoplanin, presents an MIC decrease in the magnitude of two to five orders in bacterial activity in comparison to the parent Ramoplanin. |
General description: | Rhodamine B labeled Ramoplanin is a fluorescent derivative of Ramoplanin, with a similar mode of action as Ramoplanin. It allows fluorescent labeling of Gram-positive bacteria and their detection by common fluorescent instruments. Ramoplanin is a lipoglycodepsipeptide antibiotic that is produced by the fermentation of Actinoplanes species. Fluorescent antibiotics are obtained by a synthetic conjugation of an antibiotic to a fluorophore. |
activity | specific activity: Antibiotic (bacterial staining) |
Storage Temp. | −20°C |