FITC Labeled D-Alanine
SIGMA/SBR00049 - Suitable for bacterial labeling
Empirical Formula (Hill Notation): C24H19N3O7S
Molecular Weight: 493.49
Linear Formula: C24H19N3O7S
Product Type: Chemical
E. coli cells stained with FITC labeled D-alanine. E. coli cells were harvested at mid-log phase. Pellets were washed with 1X PBS and incubated at a final concentration of 500 μM FITC labeled D-alanine solution. Bacterial growth stopped by Sodium Citrate Buffer (pH 2.25) and washed 3 times with 1X PBS. Pellets were finally reconstituted to 100 μl with 1X PBS. Images were acquired using Olympus BX53F2 upright microscope (40X2 magnification). Image depicts FITC labeled D-alanine staining, excitation/emission wavelength at 565 nm/585 nm.
Bacillus
subtilis cells stained with FITC labeled D-alanine. Bacillus subtilis cells
were harvested at mid-log phase. Pellets were washed with 1X PBS and
incubated at a final concentration of 500 μM FITC labeled D-alanine solution.
Bacterial growth stopped by Sodium Citrate Buffer (pH 2.25) and washed 3
times with 1X PBS. Pellets were finally reconstituted to 100 μl with
1X PBS. Images were acquired using Olympus BX53F2 upright
microscope (40X2 magnification). Image depicts FITC labeled D-alanine
staining, excitation/emission wavelength at 565 nm/585 nm.
subtilis cells stained with FITC labeled D-alanine. Bacillus subtilis cells
were harvested at mid-log phase. Pellets were washed with 1X PBS and
incubated at a final concentration of 500 μM FITC labeled D-alanine solution.
Bacterial growth stopped by Sodium Citrate Buffer (pH 2.25) and washed 3
times with 1X PBS. Pellets were finally reconstituted to 100 μl with
1X PBS. Images were acquired using Olympus BX53F2 upright
microscope (40X2 magnification). Image depicts FITC labeled D-alanine
staining, excitation/emission wavelength at 565 nm/585 nm.
| form | solid |
| Quality Level | 200  |
| storage temp. | −20°C |
| Analysis Note: | • Fluorescent microscopy application: Fluorescein Labeled D-Alanine has excitation/emission wavelength range at 495 nm/515 nm. • The recommended working concentration in fluorescent microscopy imaging application is between 250 μM-500 μM in DMSO. • Aliquots of the DMSO solution can be stored at -20°C, protected from light for at least one month. |
| Application: | Fluorescent labeled D-AAs can be used for many applications including: • Bacterial cell wall morphology • Bacterial cell wall formation or remodeling activity • Bacterial viability/activity • Identify bacterial activity on surfaces or in substances |
| General description: | Bacterial Peptidoglycan is a polymer consisting of sugars and amino acids, a mesh-like rigid layer that forms the cell wall. Fluorescein Labeled |
| Storage Temp. | −20°C |