FITC Labeled D-Lysine

SIGMA/SBR00050 - Suitable for bacterial labeling

Synonym: FADL; FDAA

Empirical Formula (Hill Notation): C₂₇H₂₅N₃O₇S
Molecular Weight: 535.57
Linear Formula: C₂₇H₂₅N₃O₇S
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-SBR00050-5MG		No Price

Bacillus
subtilis cells incubated with FITC labeled D-Lysine. Bacillus subtilis cells
were harvested at mid-log phase. The pellets were washed 3 times with 1X PBS.
Pellets were finally reconstituted to 100 μl with 1X
PBS. Images were acquired using Olympus BX53F2 upright microscope
(40X2 magnification). Image depicts Bacillus subtilis cells stained with FITC
labeled D-Lysine, excitation/emission wavelength at 565 nm/585 nm.

Bacillus subtilis cells incubated with FITC labeled D-Lysine. Bacillus subtilis cells were harvested at mid-log phase. The pellets were washed 3 times with 1X PBS. Pellets were finally reconstituted to 100 μl with 1X PBS. Images were acquired using Olympus BX53F2 upright microscope (40X2 magnification). Image depicts Bacillus subtilis cells stained with FITC labeled D-Lysine and DAPI.

Properties
Descriptions
Basic Data

Quality Level	200
storage temp.	−20°C

Analysis Note:	• Fluorescent microscopy application: Fluorescein Labeled D-Lysine has excitation/emission wavelength range at 495 nm/515 nm. • The recommended working concentration in fluorescent microscopy imaging application is between 250μM-500 μM in DMSO. • Aliquots of the DMSO solution can be stored at -20°C, protected from light for at least one month.
Application:	Fluorescent labeled D-AAs can be used for many applications including:• Bacterial cell wall morphology • Bacterial cell wall formation or remodeling activity • Bacterial viability/activity • Identify bacterial activity on surfaces or in substances • Differentiation betweenvarious bacterial strains according to their incorporation profile of differentD amino acids and sugars
General description:	Bacterial Peptidoglycan is a polymer consisting of sugars and amino acids, a mesh-like rigid layer that forms the cell wall. D-Amino Acids (D-AAs) are metabolically incorporated onto the bacterial cell wall by D,D-transpeptidase and/or L,D-transpeptidase. It was recently discovered that those transpeptidases catalyze the metabolic incorporation of exogenous D-AAs with almost no restriction of the side-chain identity. Modification of a D-AA with molecular fluorophores provides fluorescent D-amino acids that can efficiently label in situ peptidoglycan in diverse bacterial species.
General description:	Fluorescein Labeled D-Lysine is a fluorescent derivative of D-Lysine that is obtained by a synthetic conjugation of D-Lysine to a fluorophore. Fluorescein Labeled D-Lysine has high biocompatibility and suitability for labeling peptidoglycans in live bacteria. Additionally, it can be used in tandem with other stains such as: FITC-labelled Alanine, Rhodamine B labelled Polymyxin B, FITC labelled vancomycin and Dansyl labelled polymyxin B (SBR00049, SBR00036, SBR00028, SBR00029).

Storage Temp.

−20°C

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