Trizma® hydrochloride solution
SIGMA/SBR00051 - pH 8.5, DNase and Microbial DNA free, 10 mM
Synonym: Trizma® hydrochloride; TRIS HCl; TRIS hydrochloride; Tris(hydroxymethyl)
CAS Number: 1185-53-1
Empirical Formula (Hill Notation): C4H11NO3 · HCl
Molecular Weight: 157.60
MDL Number: MFCD00012590
Linear Formula: NH2C(CH2OH)3 · HCl
Product Type: Chemical
| concentration | 10 mM |
| InChI | 1S/C4H11NO3.ClH/c5-4(1-6, |
| InChI key | QKNYBSVHEMOAJP-UHFFFAOYSA |
| pH | 8.5 |
| pKa (25 °C) | 8.1 |
| Quality Level | 200  |
| SMILES string | Cl.NC(CO)(CO)CO |
| storage temp. | 2-8°C |
| useful pH range | 7.0-9.0 |
| Application: | The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. |
| Application: | Trizma® hydrochloride solution can be used: • As an elution buffer after PCR cleanup • As an elution buffer after DNA extraction |
| General description: | 10 mM Trizma® hydrochloride (pH 8.5) is a widely used buffer in molecular biology applications such as nucleic acid extraction and purification. No mixing or pH adjustment is necessary. Guaranteed accuracy for this buffer is ± 0.2 pH units at 25°C. |
| Legal Information: | Trizma is a registered trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | Prepared with BioUltra Trizma Base for molecular biology in 18 megaohm UV irradiated water. pH-adjusted and 0.2 μm filtered. The buffer was tested by 35 cycles of PCR for prokaryotic and eukaryotic DNA contamination. To keep the buffer contamination-free, it is recommended to use gloves and barrier-tip pipettes for dispensing. Between uses, keep the bottle closed and refrigerated, away from areas where DNA samples and PCR products may be stored. |
| Preparation Note: | Allow the buffer to reach room temperature before use. |
| Storage Temp. | 2-8°C |