SB-cyBSEP-HEK293
SIGMA/SBVT27
Synonym: Solvo membrane vesicles
Product Type: Chemical
| concentration | 5 mg/mL |
| form | liquid |
| packaging | pkg of 500 μL |
| recombinant | expressed in HEK 293 cells |
| shipped in | dry ice |
| storage temp. | −70°C |
| Application: | In the vesicular transport assay so-called "inside-out" membrane vesicles containing ABC transporters are applied. Incubating substrates of the respective efflux transporter in the presence of the inverted membrane vesicles and ATP will allow measuring accumulation of the substrates into the vesicles. In many cases radiolabeled reporter substrates are used but recently Solvo developed the new PREDIVEZ™ Vesicular Transport Kits that use fluorescent reporter substrates. The standard vesicular transport assay is an inhibitory assay performed with cold test articles. This assay provides information on any interaction between the ABC transporter and the test article. The transport of the reporter substrate is measured in the presence of the test article (typically in 7 concentrations) and IC50 is defined as the concentration inhibiting the transport of the reporter substrate by 50%. Should radiolabeled form of the investigated compound or adequate analytical methods (LC/MS, HPLC) be available, the vesicular transport assay may be performed in a direct format without the reporter substrate and may identify substrate nature of the test article. The vesicular transport substrate assay is a low throughput assay. It is suitable for low permeability test compounds as high permeability compounds may escape from the vesicles through the lipid bilayer. |
| General description: | Membrane Preparations for Vesicular Transport Assays (VT) are suitable for general drug-efflux transporter interaction studies. Both substrate and inhibitor interactions can be assessed using vesicles. The success of substrate interaction studies strongly depends on the passive permeability of the compound. High permeability substrates might not be detected. Control Membranes with no, or significantly lower transporter activity are also available. |
| Legal Information: | PREDIVEZ is a trademark of Solvo Biotechnology |
| Physical form: | Supplied as frozen membrane vesicles, containing 5 mg/mL membrane protein, labeled with volume, catalog number (transporter) and date of production. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |