Sucrose Assay Kit
SIGMA/SCA20 - sufficient for 20 assays
Synonym: Sucrose Test Kit
EC Number: 200-334-9
Product Type: Chemical
| analyte chemical class(es) | sugars (sucrose) |
| application(s) | food and beverages general analytical |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| technique(s) | photometry: suitable |
| usage | sufficient for 20 assays |
| Application: | This kit is for the quantitative, enzymatic determination of sucrose in food and other materials. Sucrose is hydrolyzed to glucose and fructose by invertase. Glucose and fructose are phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to sucrose concentration. |
| General description: | Sucrose is a disaccharide, naturally occurring carbohydrate found in plants and a major product during photosynthesis. |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-8°C |
| UNSPSC | 12164500 |
| Components | glucose assay reagent 50 mL; sucrose assay reagent 1 mL/vial; sucrose standard 500 mg |