Interleukin-6 human
SIGMA/SCU0001 - recombinant, expressed in HEK 293 cells, suitable for cell culture
Synonym: IL-6
MDL Number: MFCD00131403
Product Type: Chemical
Purity
The purity of human, recombinant Interleukin-6 (IL6) expressed in HEK 293 cells (Cat. No. SCU0001) was assessed on SDS-PAGE (2 μg/lane) followed by InstantBlue™ staining (Cat. No. ISB1L). (Representative result)
The purity of human, recombinant Interleukin-6 (IL6) expressed in HEK 293 cells (Cat. No. SCU0001) was assessed on SDS-PAGE (2 μg/lane) followed by InstantBlue™ staining (Cat. No. ISB1L). (Representative result)
Bioassay The biological activity of human, recombinant Interleukin-6 (IL6) expressed in HEK 293 cells (Cat. No. SCU0001) was measured in a cell proliferation assay using T1165 cells. The EC50 is defined as the effective concentration that elicits a 50% increase in cell growth in a cell-based bioassay.
| assay | ≥98% (SDS-PAGE) |
| biological source | human |
| color | white |
| form | lyophilized powder |
| impurities | ≤0.1 EU/μg protein (LAL test) |
| mol wt | calculated mol wt 20.8 kDa (183 amino acids glycosylated protein) |
| packaging | vial of ≥10 μg |
| potency | 0.2-2 ng/mL EC50 |
| quality | endotoxin tested |
| Quality Level | 200  |
| recombinant | expressed in HEK 293 cells |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P05231  |
| Analysis Note: | The biological activity of recombinant human IL-6 was tested in culture by measuring its ability to stimulate proliferation of the IL-6 dependent mouse T1165 cells. |
| Biochem/physiol Actions: | Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-α in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor (PCT-GF), interferon-β-2 (IFN-β2), monocyte derived human B cell growth factor, B cell stimulating factor (BSF-2), hepatocyte stimulating factor (HSF), Interleukin Hybridoma/Plasmacytoma-1 (IL-HP1). In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. |
| General description: | Interleukin 6 (IL-6) is a pleiotropic glycoprotein belonging to the IL-6 family of cytokines. IL-6 is a multifunctional cytokine with well-defined pro- and anti-inflammatory properties. IL-6 is secreted by T cells and macrophages to stimulate immune response, e.g. during infection and after trauma, especially burns or other tissue damage leading to inflammation. The O-linked sugar chain of IL-6 is thought to contribute to the stability of the cytokine through polymerization suppression and/or conformational changes, exemplifying the significance of its expression in a human expression system (which allows proper glycosylation). |
| Physical form: | This product is supplied as a lyophilized powder in phosphate buffered saline (PBS), pH 7.4, with no additives or carrier proteins. It is aseptically filled. |
| Preparation Note: | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile water to a final concentration of 100 μg/ml. Adding 0.1% recombinant human serum albumin is recommended. The product is stable for at least 2 years as supplied. After reconstitution, it is recommended to store the protein in working aliquots at –20°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |