SeqPlex RNA Amplification Kit
SIGMA/SEQR - For use with high throughput sequencing technologies
Synonym: Whole transcriptome amplification
Product Type: Chemical
Overlap between genes detected for unamplified and SeqPlex-amplified RNA. Approximately 90% of the aligned reads from SeqPlex-amplified aligned to the same Refseq genes as for unamplified.
SeqPlex library synthesis uses semi-degenerate priming plus oligo-dT for cDNA synthesis without 3’ bias. In this plot, the x-axis represents a composite of known Refseq transcripts, with distance from each respective 5’ end described as a per cent of the transcript length. This relative distance from the 5’ end, mapped against the total number of reads in the transcriptome at that position, shows similarly uniform coverage for unamplified and SeqPlex-amplified total RNA.
SeqPlex RNA Amplification kit maintains representative transcript levels during amplification. The plot above of amplified versus unamplified differential expression (human brain/UHR) shows a correlation coefficient approaching 0.9, confirming the capability of the SeqPlex RNA amplification kit to perform with minimal bias.
SEQR WTA Procedure
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | whole genome amplification: suitable |
| Application: | SeqPlex™ RNA Amplification Kit has been used to amplify poly(A)-selected RNA. |
| Features and Benefits: | • Low quantities of total RNA random priming technology amplifies fragmented or intact RNA from all sources including FFPE and RIP. • Semi-degenerate library primer design for more complete transcriptome coverage and efficient priming. • No need to fragment DNA before sequencing. • Amplifies ds-cDNA in 8 hours or less. • Compatible with all next generation sequencing platforms except Pacific Bioscience. |
| General description: | The SeqPlex™ RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow. It also facilitates the amplification of non-polyA tailed RNA isolated from tissue, cultured cells, formalin-fixed samples, or serum while maintaining patterns of differential expression found in the unamplified sample. |
| Legal Information: | SeqPlex is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | SEQR-500RXN is manufactured on-demand. Contact us for more information. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |