SeqPlex RNA Amplification Kit
SIGMA/SEQR - For use with high throughput sequencing technologies
Synonym: Whole transcriptome amplification
Product Type: Chemical
Quality Level | 200 ![]() |
shipped in | wet ice |
storage temp. | −20°C |
technique(s) | whole genome amplification: suitable |
Application: | SeqPlex™ RNA Amplification Kit has been used to amplify poly(A)-selected RNA. |
Features and Benefits: | • Low quantities of total RNA random priming technology amplifies fragmented or intact RNA from all sources including FFPE and RIP. • Semi-degenerate library primer design for more complete transcriptome coverage and efficient priming. • No need to fragment DNA before sequencing. • Amplifies ds-cDNA in 8 hours or less. • Compatible with all next generation sequencing platforms except Pacific Bioscience. |
General description: | The SeqPlex™ RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow. It also facilitates the amplification of non-polyA tailed RNA isolated from tissue, cultured cells, formalin-fixed samples, or serum while maintaining patterns of differential expression found in the unamplified sample. |
Legal Information: | SeqPlex is a trademark of Sigma-Aldrich Co. LLC |
Other Notes: | SEQR-500RXN is manufactured on-demand. Contact us for more information. |
RIDADR | NONH for all modes of transport |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | −20°C |
UNSPSC | 12352200 |