MISSION® pLKO.1-puro TurboGFP™ shRNA Control Plasmid DNA
SIGMA/SHC004 - shRNA sequence targeting tGFP
Synonym: MISSION® Control Vectors
MDL Number: MFCD07785395
Product Type: Chemical
TRC1 shRNA to TurboGFP Control Vector
| concentration | 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) |
| product line | MISSION® |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| Application: | MISSION® pLKO.1-puro TurboGFP™ shRNA control plasmid DNA has been used as lentiviral transduction and RNA interference assay. |
| Application: | To see more application data, protocols, vector maps visit sigma.com/shrna . |
| General description: | The MISSION TurboGFP shRNA Control Vector is a 7,087 base pair lentivirus plasmid vector that contains an shRNA sequence targeting TurboGFP. The TurboGFP shRNA Control Vector is useful as a positive knockdown control in experiments using the MISSION TurboGFP positive control vector or in cell lines expressing TurboGFP. TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The TurboGFP shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl. |
| Legal Information: | MISSION is a registered trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | TurboGFP is a trademark of Evrogen Co. |
| Legal Information: | Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/m. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |