MISSION® pLKO.1-puro eGFP shRNA Control Plasmid DNA
SIGMA/SHC005 - shRNA sequence targeting eGFP
Synonym: MISSION® Control Vectors
MDL Number: MFCD07785395
Product Type: Chemical
shRNA to eGFP Control Vector
| concentration | 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) |
| product line | MISSION® |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| Application: | To see more application data, protocols, vector maps visit sigma.com/shrna . |
| General description: | The MISSION® eGFP shRNA Control Vector is a 7,091 base pair lentivirus plasmid vector that contains an shRNA sequence targeting eGFP (GenBank Accession No. pEGFP U55761). The eGFP shRNA Control Vector is useful as a positive knockdown control in experiments using cell lines expressing eGFP. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids, MISSION® Lentiviral Packaging Mix (Prod. No. SHP001). The eGFP shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl. |
| Legal Information: | MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/m. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106609 |