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MISSION® pLKO.1-puro eGFP shRNA Control Transduction Particles

SIGMA/SHC005V - shRNA sequence targeting eGFP

Synonym: MISSION®; MISSION® Control Transduction Particles

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-SHC005V 1 pkg
$390.00
1/EA
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shRNA to eGFP Control Vector

 

concentration ≥1x106 VP/ml (via p24 assay)
product line MISSION®
Quality Level 200 
shipped in dry ice
storage temp. −70°C
technique(s) capture ELISA: 106 TU/mL using p24
Application: To see more application data, protocols, vector maps visit sigma.com/shrna .
General description: The MISSION eGFP shRNA Control Transduction Particles contain an shRNA sequence targeting eGFP (GenBank Accession No. pEGFP U55761). The eGFP shRNA Control Particles are useful as a positive knockdown control in experiments using cell lines expressing eGFP.
The eGFP shRNA Control Transduction Particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-eGFP shRNA (Prod. No. SHC005). Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the control transduction particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
General description: When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna .
Legal Information: MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
RIDADR UN 3245 9
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −70°C
UNSPSC 12352200

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