MISSION® pLKO.1-puro Luciferase shRNA Control Plasmid DNA
SIGMA/SHC007 - shRNA sequence targeting luciferase
Synonym: MISSION® Control Vectors
MDL Number: MFCD07785395
Product Type: Chemical
concentration | 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) |
product line | MISSION® |
Quality Level | 200 ![]() |
shipped in | dry ice |
storage temp. | −20°C |
Application: | To see more application data, protocols, vector maps visit sigma.com/shrna ![]() |
General description: | The MISSION® Luciferase shRNA Control Vector is a 7,091 base pair lentivirus plasmid vector that contains an shRNA sequence targeting luciferase from Photinus pyralis (GenBank Accession No. M15077). The Luciferase shRNA Control Vector is useful as a positive knockdown control in experiments using cell lines expressing firefly luciferase. It can also be used as a negative control vector. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids, MISSION® Lentiviral Packaging Mix (Prod. No. SHP001). The Luciferase shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl. |
Legal Information: | MISSION is a registered trademark of Sigma-Aldrich Co. LLC |
Legal Information: | Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/m![]() |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | −20°C |
UNSPSC | 12352200 |