MISSION® pLKO.1-puro Luciferase shRNA Control Transduction Particles
SIGMA/SHC007V - shRNA sequence targeting luciferase
Synonym: MISSION®; MISSION® Control Transduction Particles
Product Type: Chemical
shRNA to Luciferase Control Vector
| concentration | ≥1x106 VP/ml (via p24 assay) |
| product line | MISSION® |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −70°C |
| technique(s) | capture ELISA: 106 TU/mL using p24 |
| Application: | To see more application data, protocols, vector maps visit sigma.com/shrna . |
| General description: | The MISSION Luciferase shRNA Control Transduction Particles contain an shRNA sequence targeting Luciferase, Photinus pyralis (GenBank Accession No. M15077). The Luciferase shRNA Control Particles are useful as a positive knockdown control in experiments using cell lines expressing firefly luciferase. The Luciferase shRNA control transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-luciferase shRNA (Prod. No. SHC007). Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the control transduction particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock. |
| General description: | When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency. To see more application data, protocols, vector maps visit sigma.com/shrna . |
| Legal Information: | MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany |
| RIDADR | UN 3245 9 |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |