MISSION® pLKO.1-puro-CMV-TagRFP™ Positive Control Transduction Particles
SIGMA/SHC012V - Contains a gene encoding TagRFP
Synonym: MISSION®; MISSION® Control Transduction Particles
Product Type: Chemical
TagRFP Control Vector
| concentration | ≥1x106 VP/ml (via p24 assay) |
| product line | MISSION® |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −70°C |
| technique(s) | capture ELISA: 106 TU/mL using p24 |
| Application: | MISSION® pLKO.1-puro-CMV-TagRFP™ Positive Control Transduction Particles has been used to knockdown transient receptor potential vanilloid 1 receptor (TRPV1)-like receptors. It has also been used to generate fluorescent cell lines. |
| Application: | MISSION®pLKO.1-puro-CMV-TagRFP™ Positive Control Transduction Particles has been used in generating fluorescent cell lines by transduction. It has also been used in transfection of human dermal fibroblast (HDF), WI-38 and IMR-90 FBs to express tagRFP. |
| Application: | To see more application data, protocols, vector maps visit sigma.com/shrna . |
| Application: | When conducting experiments using MISSION shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION TagRFP Control Transduction Particles are a positive control to monitor transduction efficiency. The MISSION TagRFP Control Transduction Particles are produced from the sequence-verified 8594 base pair lentivirus plasmid, pLKO.1-puro-CMV-TagRFP (SHC012). This construct aids in interpretation of experimental design and results by providing a red fluorescent protein marker for monitoring success in transduction of your cells of interest. |
| General description: | Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock. |
| General description: | When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency. To see more application data, protocols, vector maps visit sigma.com/shrna . |
| Legal Information: | MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | TagRFP is a trademark of Evrogen Co. |
| RIDADR | UN 3245 9 |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |