MISSION® pLKO.1-puro-UbC-TurboGFP™ Positive Control Plasmid DNA
SIGMA/SHC014 - Contains a gene encoding TurboGFP, under the control of the UbC promoter
Synonym: MISSION® Control Vectors
MDL Number: MFCD07785395
Product Type: Chemical
concentration | 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) |
product line | MISSION® |
Quality Level | 200 |
shipped in | dry ice |
storage temp. | −20°C |
Application: | To see more application data, protocols, vector maps visit sigma.com/shrna . Silencing of the CMV promoter can be a problem in some cell types. For these cells, the ubiquitin promoter (UbC) can be a viable alternative. The MISSION UbC-TurboGFP Control Vector is a 9092 base pair lentivirus plasmid vector that contains a gene encoding TurboGFP, under the control of the UbC promoter. The UbC-Turbo-GFP Control Vector is useful as a positive control in experiments using the MISSION shRNA library clones for applications in which an alternate promoter is necessary. |
General description: | Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, we recommend producing self-inactivating replication incompetent viral particles in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The UbC-TurboGFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl. |
Legal Information: | MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany |
Legal Information: | TurboGFP is a trademark of Evrogen Co. |
Legal Information: | Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/m |
RIDADR | NONH for all modes of transport |
WGK Germany | nwg |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | −20°C |
UNSPSC | 12352200 |