MISSION® pLKO.1-puro-UbC-TurboGFP™ Positive Control Transduction Particles
SIGMA/SHC014V - Contains a gene encoding TurboGFP, under the control of the UbC promoter
Synonym: MISSION®; MISSION® Control Transduction Particles
Product Type: Chemical
UbC-TurboGFP Control Vector
| concentration | ≥1x106 VP/ml (via p24 assay) |
| product line | MISSION® |
| Quality Level | 100  |
| 200 |
|
| shipped in | dry ice |
| storage temp. | −70°C |
| technique(s) | capture ELISA: 106 TU/mL using p24 |
| Application: | To see more application data, protocols, vector maps visit sigma.com/shrna . |
| Application: | When conducting experiments using MISSION shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. Silencing of the CMV promoter can be a problem in some cell types. For these cells, the ubiquitin promoter (UbC) can be a viable alternative. The MISSION UbC-TurboGFP Control Transduction Particles are a positive control to monitor transduction efficiency for applications in which an alternate promoter is necessary. The MISSION UbC-TurboGFP Control Transduction Particles are produced from the sequence-verified 9092 base pair lentivirus plasmid, pLKO.1-puro-UbC-TurboGFP (SHC014). This construct aids in interpretation of experimental design and results by providing a green fluorescent protein marker for monitoring success in transduction of your cells of interest. |
| General description: | Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock. |
| General description: | When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency. To see more application data, protocols, vector maps visit sigma.com/shrna . |
| Legal Information: | MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | TurboGFP is a trademark of Evrogen Co. |
| RIDADR | UN 3245 9 |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |