MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles, High Titer
SIGMA/SHC016H - Targets no known genes from any species
Synonym: MISSION®; MISSION® Control Transduction Particles; negative control; negative shRNA control; non-target control; non-target shRNA; non-target shRNA control; shRNA control
Product Type: Chemical
| concentration | ≥1x109 VP/ml (via p24 assay) |
| product line | MISSION® |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −70°C |
| Application: | To see more application data, protocols, vector maps visit sigma.com/shrna . |
| General description: | The MISSION pLKO.1-puro Non-Target shRNA Control Transduction Particles, High Titer contain an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION shRNA library clones. This allows one to examine the effect of transduction of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. High titer virus is useful when performing in vivo research. |
| General description: | When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency. To see more application data, protocols, vector maps visit sigma.com/shrna . |
| Legal Information: | MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany |
| RIDADR | UN 3245 9 |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |