MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles
SIGMA/SHC016V - Targets no known genes from any species
Synonym: MISSION®; MISSION® Control Transduction Particles; negative control; negative shRNA control; non-target control; non-target shRNA; non-target shRNA control; shRNA control
Product Type: Chemical
concentration | ≥1x106 VP/ml (via p24 assay) |
product line | MISSION® |
Quality Level | 100 |
200 | |
shipped in | dry ice |
storage temp. | −70°C |
Analysis Note: | To see more application data, protocols, vector maps visit sigma.com/shrna . |
Application: | MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles have been used to generate 3T3-L1 (pre-adipocytes) control cell lines. |
General description: | The MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles contain an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION® shRNA library clones. This allows one to examine the effect of transduction of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Transduction Particles are provided as 200 μL at 1 x 106 TU/mL via p24 assay. |
General description: | When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency. To see more application data, protocols, vector maps visit sigma.com/shrna . |
Legal Information: | MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany |
RIDADR | UN 3245 9 |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | −70°C |
UNSPSC | 12352200 |