MISSION® 1X LacO Inducible Non-Target shRNA Control Plasmid DNA
SIGMA/SHC312 - Does not target any known genes
Synonym: MISSION® Control Vectors
MDL Number: MFCD07785395
Product Type: Chemical
| concentration | 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) |
| product line | MISSION® |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| Application: | To see more application data, protocols, vector maps visit sigma.com/shrna . |
| General description: | Sigma® is proud to offer IPTG-inducible vectors as the latest development from our continued partnership in TRC. The pLKO vector has been redesigned to contain a LacI (repressor) and a modified human U6 shRNA promoter with LacO (operator) sequences. In the absence of IPTG (isopropyl-Β-D-thio-galac The vector contains an shRNA insert that does not target any known genes, making it useful as a negative control in experiments using the MISSION inducible shRNA library clones. This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The 1X LacO Inducible Non-Target shRNA Control is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl. |
| Legal Information: | MISSION is a registered trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | Sigma is a registered trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/m. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |