MISSION® 1X LacO Inducible Non-Target shRNA Control Transduction Particles
SIGMA/SHC312V - Does not target any known genes
Product Type: Chemical
| concentration | ≥1x106 VP/ml (via p24 assay) |
| product line | MISSION® |
| Quality Level | 100  |
| 200 |
|
| shipped in | dry ice |
| storage temp. | −70°C |
| Application: | To see more application data, protocols, vector maps visit sigma.com/shrna . |
| General description: | Sigma® is proud to offer IPTG-inducible vectors as the latest development from our continued partnership in TRC. The pLKO vector has been redesigned to contain a LacI (repressor) and a modified human U6 shRNA promoter with LacO (operator) sequences. In the absence of IPTG (isopropyl-Β-D-thio-galac The MISSION 1x LacO Inducible Non-Target shRNA Control Transduction Particles contain an shRNA insert that does not target any known genes, making it useful as a negative control in experiments using the MISSION inducible shRNA library clones. This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. 200uL of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock. When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. |
| Legal Information: | MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | Sigma is a registered trademark of Sigma-Aldrich Co. LLC |
| RIDADR | UN 3245 9 |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |