MISSION® shRNA
SIGMA/SHCLNV - Lentiviral Particles
Product Type: Chemical
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| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −70°C |
| technique(s) | capture ELISA: 106 VP/mL using p24 |
| Application: | MISSION® shRNA has been used: • in transfections and transductions • in the establishment of stable cell lines expressing shRNA • in the generation of alpha-actinin-4 (ACTN4) knockdown cell line • to transduce raw 264.7 cells |
| General description: | Lentiviral particles are ideal for transducing a wide range of cell lines. VSV-G Psuedotyping of the MISSION® lentiviral particles allows for entry into a wide range of cells. In addition, lentiviral particles stably integrate the shRNA into the genomes of both dividing and non-dividing cell lines. The MISSION bioproduction team regularly produces high quality lentivirus, so that our customers can get straight to their RNAi experiment without wasting precious time on tedious lentiviral production. Our minimum titer is 106 VP/mL. Unlike adenoviral infections that require vast excesses, lentiviral transduction is efficient enought allow for MOI as low as 1. |
| General description: | Our comprehensive shRNA product offering consists of over 150,000 pre-cloned shRNA constructs targeting more than 15,000 human and 15,000 mouse genes. The library is available in: gene family sets: gene collections related to specific functional classes such as kinases and ion channels; gene taget sets, sets of shRNA clones that target your favorite gene; and individual shRNA clones. Our shRNA and lentiviral manufacturing platforms utilize dedicated laboratories featuring state-of-the-art robotics, liquid handling and laboratory information management systems (LIMS) to provide superior quality and service for your RNAi research needs. |
| Legal Information: | MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | Use of this product is subject to one or more license agreements. |
| Other Notes: | To see protocols and application data please visit sigma.com/shrna . |
| RIDADR | UN 3245 9 |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −70°C |
| UNSPSC | 41106609 |