CRISPR & MISSION® Lentiviral Packaging Mix
SIGMA/SHP002
Product Type: Chemical
| application(s) | CRISPR |
| concentration | 100 ng/μL (in Tris-EDTA) |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| Application: | Functional Genomics/Screening/Target Validation |
| General description: | The CRISPR & MISSION Lentiviral Packaging Mix is an optimized formulation of two plasmids expressing the key HIV packaging genes and a heterologous viral envelope gene. Lentiviral particles are generated from three main components: • The packaging vector, which contains the minimal set of lentiviral genes required to generate the virion structural proteins and packaging functions. • The vesicular stomatitis virus G-protein envelope vector, which provides the heterologous envelope for pseudotyping and allows these lentiviral particles to efficiently deliver the transfer sequence of interest to a wide variety of cell types, including primary and non-dividing cells • The lentiviral transfer vector, which contains the sequence of interest as well as the cis acting sequences necessary for packaging. The Lentiviral Packaging Mix contains the first two components; it is designed to be co-transfected along with a compatible lentiviral transfer vector in order to create high-titer pseudo-typed lentiviral particles used for downstream transduction applications. This product is recommended for packaging Lenti CRISPR, Lenti ORF, and Lenti shRNA vectors. |
| Legal Information: | CRISPR Use License Agreement |
| Legal Information: | MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |