MISSION® LentiPlex® Human Pooled shRNA Library
SIGMA/SHPH01 - For Rapid, Convenient Genome-wide shRNA Screens
Synonym: Human shRNA Library; LentiPlex shRNA Library
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| concentration | ≥5x108 VP/ml (via p24 assay) |
| product line | MISSION® |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −70°C |
| technique(s) | capture ELISA: 5 × 108 TU/mL using p24 |
| Application: | MISSION® LentiPlex® Human Pooled shRNA Library has been used to transfect human colon adenocarcinoma cell line HT29 for shRNA screening and bioinformatics analysis. |
| General description: | MISSION LentiPlex Pooled Libraries bring the power of the MISSION TRC shRNA collection together with Sigma¢s lentiviral manufacturing expertise to enable genome-wide RNAi screens at your bench-top. The LentiPlex pooled system provides enhanced delivery and long-term gene silencing in non-dividing and primary cell lines allowing unlimited discovery potential. The convenient format now makes rapid whole-genome RNAi screens accessible to any researcher with minimal reagent, time or capital equipment investment. The MISSION LentiPlex Human shRNA Pooled Library is a genome-wide lentiviral pool produced using a proprietary process. The library consists of over 75,000 shRNA constructs from the TRC collection targeting 15,000+ human genes. Each library is tested for shRNA representation before product release to ensure robust library coverage. The library is provided in ready-to-use lentiviral format at titers of at least 5 x 108 TU/ml via p24 assay and is pre-divided into ten subpools of approximately 8,000 shRNA constructs each. Amplification and sequencing primers are also provided for downstream target identification. The library is pre-divided into ten subpools of approximately 8,000 shRNA constructs each. Each library is enough material for six screens at 100X representation. Please visit sigma.com/lentiplex to learn more and see example data and uses. |
| Legal Information: | Lentiviral License Agreement |
| Legal Information: | LentiPlex is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Deconvolution of shRNA Pools Sigma′s deconvolution service lets you easily identify the genes that impact your pooled shRNA screen.• Next-generation sequencing of clones gives a precise number of individual clone occurrence within a pooled shRNA sample • Comprehensive, reproducible results from pooled shRNA screens • Statistically robust and information-rich data Contact your local Sigma sales representative for more information or submit an inquiry to MISSION RNAi  |
| RIDADR | UN3373 - class 6.2 Biological substance, Category B |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −70°C |
| UNSPSC | 41105904 |