Sialic Acid Quantitation Kit
SIGMA/SIALICQ - Sufficient for 25 reactions
Product Type: Chemical
Quality Level | 200 |
shipped in | wet ice |
storage temp. | 2-8°C |
Application: | Contains all reagents to quantitate the amount of N-acetylneuraminic acid, either free, or in glycoproteins, cell surface glycoproteins, polysialic acids and capsular polysaccharides (consisting only of polysialic acid). |
General description: | The Sialic Acid Quantitation Kit provides a rapid and accurate determination of total N-acetylneuraminic acid (NANA; sialic acid). NANA is released from glycoconjugates using neuraminidase. The kit uses α(2→3,6,8,9) neuraminidase to cleave all NANA linkages, including α(2→8) and α(2→9) linkages, as well as branched NANA, for the most accurate determination of total sialic acid content. |
Quantity: | Sufficient reagents are provided to perform 25 reactions, including control samples. Each reaction will measure 1-200 nmoles of NANA. |
RIDADR | NONH for all modes of transport |
Storage Temp. | 2-8°C |
UNSPSC | 41116107 |
Components | N-Acetylneuraminic acid aldolase 25 μL; N-Acetylneuraminic acid 200 μL; α(2→3,6,8,9)-Neuraminidase 25 μL; Fetuin, bovine .5 mg; Lactic dehydrogenase 25 μL; β-NADH disodium salt 3 vial(s); Tris-HCl, pH 7.5 1 mL; Sialidase buffer 500 μL |