Synonym: N-6-[2-[[4-(2,4-Dichlorophenyl)-5-(1H-imidazol-1-yl)-2-pyrimidinyl]amino]ethyl]-3-nitro-2,6-pyridinediamine; CHIR-98014
CAS Number: 252935-94-7
Empirical Formula (Hill Notation): C20H17Cl2N9O2
Molecular Weight: 486.31
MDL Number: MFCD10565922
Linear Formula: C20H17Cl2N9O2
Product Type: Chemical
| assay |
≥98% (HPLC) |
| color |
white to brown |
| form |
powder |
| InChI |
1S/C20H17Cl2N9O2/c21-12-1-2-13(14(22)9-12)18-16(30-8-7-24-11-30)10-27-20(29-18)26-6-5-25-17-4-3-15(31(32)33)19(23)28-17/h1-4,7-11H,5-6H2,(H3,23,25,28)(H,26,27,29) |
| InChI key |
MDZCSIDIPDZWKL-UHFFFAOYSA-N |
| Quality Level |
200  |
| SMILES string |
NC1=C(C=CC(NCCNC2=NC(C3=C(Cl)C=C(Cl)C=C3)=C(N4C=CN=C4)C=N2)=N1)[N+]([O-])=O |
| solubility |
DMSO: 0.5 mg/mL, clear (warmed) |
| storage temp. |
−20°C |
| Application: |
CHIR 98014 has been used for the generation of small molecules neural progenitor cells and differentiation towards motor neurons. It has also been used as a Wnt/β-catenin pharmacological agonist in the cell-conditioned medium to perform chromatin immunoprecipitation (ChIP) studies in HT22 neurons. |
| Biochem/physiol Actions: |
CHIR 98014 is a glycogen synthase kinase-3 (GSK-3) inhibitor with IC50 values of 0.65 nM and 0.58 nM for GSK3α and GSK3β, respectively. CHIR98014 is 500-fold to >10,000-fold selectivity for GSK-3 versus 20 other protein kinases tested, including closest homologs Cdc2 and ERK2. |
| Biochem/physiol Actions: |
CHIR 98014 is a glycogen synthase kinase-3 (GSK-3) inhibitor. |
| Packaging: |
5, 25 mg in glass bottle |
| Preparation Note: |
Reconstitute in DMSO at 0.5 mg/mL. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. |
| Preparation Note: |
The product in the original sealed container is quite stable. It should be stored at −20°C in the powdered format. |
| Hazard Codes |
Xn |
| Risk Statements |
22 |
| RIDADR |
NONH for all modes of transport |
| WGK Germany |
WGK 3 |
| Flash Point(F) |
Not applicable |
| Flash Point(C) |
Not applicable |
| Purity |
≥98% (HPLC) |
| Storage Temp. |
−20°C |
| UNSPSC |
12352200 |
