7-AAD Ready Made Solution
SIGMA/SML1633 - 1 mg/mL
Synonym: 7-Aminoactinomycin D Ready Made Solution; 7-Aminodactinomycin Ready Made Solution
CAS Number: 7240-37-1
Empirical Formula (Hill Notation): C62H87N13O16
Molecular Weight: 1270.43
Linear Formula: C62H87N13O16
Product Type: Chemical
| concentration | 1 mg/mL |
| form | liquid (solution in DMSO:H2O (1:1)) |
| InChI | 1S/C62H87N13O16/c1-26(2)4 |
| InChI key | YXHLJMWYDTXDHS-UHFFFAOYSA |
| Quality Level | 200  |
| SMILES string | N21C(CCC2)C(=O)N(CC(=O)N( |
| storage condition | protect from light |
| storage temp. | −20°C |
| Application: | Fluorescent DNA stain. A featured product for Apoptosis research. |
| Biochem/physiol Actions: | 7-AAD is used in flow cytometry analysis of viable cells. 7-ADD is a fluorescent indicator that undergoes a spectral shift upon association with DNA. Su, X., et al. used FITC and PE-conjugated antibodies to stain cell surface markers. After cell-surface marker staining, cells were further stained with 10 μg/ml of 7-AAD in PBS on ice for 30 minutes. After washing with PBS twice, the cells were fixed in 1% paraformaldehyde supplemented with 50 μg/ml actinomycin D. Non-apoptotic cells were 7-AAD negative. 7-AAD like its parent molecule, Actinomycin D, is a DNA-intercalator with growth-inhibitory properties. |
| Biochem/physiol Actions: | 7-amino-actinomycin D (7-AAD) is a DNA dye. It can be used as a replacement for propidium iodide (PI) for the discrimination of dead cells. This dye is ideal for dead cell discrimination in long-lasting experiments, as it is efficiently exempted by intact cells and has a high DNA binding constant. |
| General description: | Free form: Excitation: 503 nm (0.01 M phosphate buffer, pH 7.0 containing 0.1 mM EDTA); 550 nm Emission: 675 nm (0.01 M phosphate buffer, pH 7.0 containing 0.1 mM EDTA); 672 nm DNA Complex: Excitation: 543 nm; 555 nm Emission: 655 nm; 665 nm |
| Preparation Note: | Solution of 1 mg/ml in DMSO:H2O (1:1) is stable for at least 2 years at −20 °C without loss of activity. According to Su et al., the provided solution should be diluted to 10 μg/ml (1:100) working concentration in PBS and is therefore sufficient for 100 stain reactions. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |