Bafilomycin A1 Ready Made Solution
SIGMA/SML1661 - 0.16 mM in DMSO, from Streptomyces griseus
CAS Number: 88899-55-2
Empirical Formula (Hill Notation): C35H58O9
Molecular Weight: 622.83
Linear Formula: C35H58O9
Product Type: Chemical
| biological source | Streptomyces griseus |
| concentration | 0.16 mM in DMSO |
| form | DMSO solution |
| InChI | 1S/C35H58O9/c1-19(2)32-24 |
| InChI key | XDHNQDDQEHDUTM-JQWOJBOSSA |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| Application: | Bafilomycin A1 has been used:• as an endosome acidification inhibitor to study the importance of endosome acidification in the extracellular vesicle uptake and cytosolic release of stably expressing NanoLuc luciferase-tagged Hsp70 (NLuc-Hsp70) in HeLa cells • as a vacuolar-type H+-ATPase (V-ATPase) inhibitor to study its effects on autophagic turnover of light chain 3 β (LC3-II) in mice • as an autophagy inhibitor to study its effects on primary rat liver sinusoidal endothelial cells (LSECs) defenestration |
| Biochem/physiol Actions: | Bafilomycin A1 belongs to the pleomacrolides family and it inhibits autophagy. It may exhibit anti-tumorigenic, anti-parasitic, and anti-neurodegenerative effects. Bafilomycin A1, found in lysosomes and endosomes prevents the acidification of these cell organelles. It also participates in blocking autophagosome-lysosome fusion and autolysosome acidification, steps necessary for maintaining the autophagic flux and cellular homeostasis. |
| Biochem/physiol Actions: | Bafilomycin A1 is a macrolide antibiotic. Bafilomycin A1 acts as a potent and selective inhibitor of vacuolar-type H+-ATPase. |
| Other Notes: | Bafilomycin A1 solution is provided in concentration of 0.16 mM. Typical concentrations for use in cell culture are 50-100 nM. |
| WGK Germany | WGK 1 |
| Flash Point(F) | 188.6 °F - closed cup |
| Flash Point(C) | 87 °C - closed cup |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |