4-Hydroxytamoxifen Ready Made Solution
SIGMA/SML1666 - 5 mg/mL in ethanol: isopropanol (95:5)
Synonym: 4-OHT; cis/trans-4-Hydroxytamoxifen
CAS Number: 68392-35-8
Empirical Formula (Hill Notation): C26H29NO2
Molecular Weight: 387.51
Linear Formula: C26H29NO2
Product Type: Chemical
| biological source | synthetic |
| concentration | 5 mg/mL in ethanol: isopropanol (95:5) |
| form | solution |
| InChI | 1S/C26H29NO2/c1-4-25(20-8 |
| InChI key | TXUZVZSFRXZGTL-QPLCGJKRSA |
| Quality Level | 200  |
| shipped in | dry ice |
| SMILES string | N(CCOc1ccc(cc1)C(=C(CC) |
| storage temp. | −20°C |
| Biochem/physiol Actions: | 4-Hydroxytamoxifen is an active metabolite of Tamoxifen. |
| Biochem/physiol Actions: | 4-Hydroxytamoxifen is an active metabolite of Tamoxifen. 4-Hydroxytamoxifen is formed by cytochrome P450 2D6 in human liver and is a potent and selective estrogen receptor antagonist. 4-Hydroxytamoxifen has been used to stimulate LC3 lipidation and to form autophagic vesicles in a superoxide-dependent manner. Tamoxifen and 4-Hydroxytamoxifen markedly induce cytochrome P450 3A4 (a major drug-metabolizing enzyme) in primary cultures of human hepatocytes. 4-Hydroxytamoxifen undergoes a cis-trans (E-Z) isomerization, a process that occurs in all common laboratory solvents. |
| Biochem/physiol Actions: | Trans isoform of 4-hydroxytamoxifen possesses antiestrogenic activity, while the cis 4-hydroxytamoxifen serves as a key agonist for estrogen receptor. 4-Hydroxytamoxifen promotes apoptosis via activation of p38 pathway in breast cancer cell cultures. |
| Preparation Note: | 4-Hydroxytamoxifen is provided in a 13 mM solution. The recommended working concentration is 10-100 μM. Therefore, 4-Hydroxytamoxifen Ready Made Solution should be diluted 1:130 - 1:1,300 in cell culture media. |
| Symbol |    GHS02,GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H225 - H319 - H350 - H360FD - H412 |
| Precautionary statements | P201 - P210 - P273 - P305 + P351 + P338 - P308 + P313 |
| RIDADR | UN1170 - class 3 - PG 2 - Ethanol, solution |
| WGK Germany | WGK 3 |
| Flash Point(F) | 57.2 °F |
| Flash Point(C) | 14 °C |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |