FHZ
SIGMA/SML1734 - ≥98% (HPLC)
Synonym: 2-Amino-3′-hydroxy-6′-(2-(2-(2-hydroxyethoxy)ethoxy)ethoxy)spiro[isoindoline-1,9′-xanthen]-3-one
CAS Number: 1883737-63-0
Empirical Formula (Hill Notation): C26H26N2O7
Molecular Weight: 478.49
Linear Formula: C26H26N2O7
Product Type: Chemical
| assay | ≥98% (HPLC) |
| color | white to light brown |
| form | powder |
| Quality Level | 100  |
| SMILES string | OC1=CC(OC(C=C(OCCOCCOCCO) |
| solubility | DMSO: 30 mg/mL, clear |
| storage condition | protect from light |
| storage temp. | 2-8°C |
| Biochem/physiol Actions: | FHZ is a highly selective, cell-permeable, non-cytotoxic, non-fluorescent 6-triethylene glycol-substituted fluorescein hydrazide that is an efficient fluorescent turn-on probe for dual channel detection of (•OH) and HOCl in live cells and zebrafish embryos. FHZ selectively reacts with hydroxyl (•OH) and HOCl, respectively, to form highly fluorescent FOBA (Ex: 410 nm & Em.: 486 nm; Φ = 0.42; ε410 = 12000/M/cm) and F-TEG (Ex: 490 nm & Em.: 520 nm; Φ = 0.34; ε490 = 7000/M/cm), while exhibiting no reactivity toward H2O2 1O2, O2•–, NO, or ONOO–. Cyan and green fluorescence dual-channel detection with two exciting wavelengths (405 nm and 488 nm) and two collection windows (430–490 nm; 510–560 nm) allows time-dependent quantitative detection of cellular •OH and HOCl by FHZ in live cells (50-100 μM; HeLa and RAW 264.7 macrophages) cells and zebrafish embryos (50 μM). |
| Packaging: | 25 mg in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |