PFM01

SIGMA/SML1735 - ≥98% (HPLC)

Synonym: (5Z)-5-[(4-Hydroxyphenyl)methylene]-3-(2-methylpropyl)-2-thioxo-4-thiazolidinone; 5-(4-Hydroxybenzylidene)-3-isobutyl-2-thioxothiazolidin-4-one

CAS Number: 1558598-41-6
Empirical Formula (Hill Notation): C₁₄H₁₅NO₂S₂
Molecular Weight: 293.40
MDL Number: MFCD02220972
Linear Formula: C₁₄H₁₅NO₂S₂
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-SML1735-5MG	5 mg	$114.00 1/EA	Add To Favorites
45-SML1735-25MG	25 mg	$464.00 1/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

assay	≥98% (HPLC)
color	white to beige
form	powder
Quality Level	100
SMILES string	OC1=CC=C(/C=C2SC(N(CC(C)C)C2=O)=S)C=C1
solubility	DMSO: 20 mg/mL, clear
storage temp.	2-8°C

Biochem/physiol Actions:

PFM01 is a cell-permeable N-alkylated Mirin (Sigma Cat. No. 475954) derivative that selectively inhibits against MRE11 endo-, but not exo-, nuclease activity. PFM01 targets MRE11 at a site near the dimer interface, distinct from that occupied by Mirin and PFM39 to allow disruption of the ssDNA-binding groove and selective inhibition against MRE11 endo-, but not exo-, nuclease activity. While both endonuclease and exonuclease activities are required for MRE11-mediated homologous recombination (HR) repair, only FM01 (100 μM), but not the exonuclease inhibitors Mirin (500 μM) and PFM39 (100 μM), rescues G2-phase double-strand break (DSB) repair defect in HR protein BRCA2-deficient HSC62-hTERT fibroblasts following ionizing irradiation (IR) by blocking HR initiation and thereby allowing non-homologous end joining (NHEJ) to proceed.

Packaging:

5, 25 mg in glass bottle

Symbol	GHS07
Signal word	Warning
Hazard statements	H302 - H315 - H319
Precautionary statements	P301 + P312 + P330 - P305 + P351 + P338
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥98% (HPLC)
Storage Temp.	2-8°C
UNSPSC	12352200

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