Synonym: (3aS,4S,6aR)-Hexahydro-N-[2-(4-hydroxyphenyl)ethyl]-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide; BP; Biotin-Phenol
CAS Number: 41994-02-9
Empirical Formula (Hill Notation): C18H25N3O3S
Molecular Weight: 363.47
MDL Number: MFCD27953026
Linear Formula: C18H25N3O3S
Product Type: Chemical
| assay |
≥97% (HPLC) |
| color |
white to beige |
| form |
powder |
| InChI |
1S/C18H25N3O3S/c22-13-7-5-12(6-8-13)9-10-19-16(23)4-2-1-3-15-17-14(11-25-15)20-18(24)21-17/h5-8,14-15,17,22H,1-4,9-11H2,(H,19,23)(H2,20,21,24)/t14-,15-,17-/m0/s1 |
| InChI key |
VZWXNOBHWODXCW-ZOBUZTSGSA-N |
| SMILES string |
O=C(NCCC1=CC=C(O)C=C1)CCCC[C@@H]2SC[C@@]3([H])[C@]2([H])NC(N3)=O |
| solubility |
DMSO: 2 mg/mL, clear |
| storage temp. |
−20°C |
| Application: |
Biotinyl tyramide or Biotin-Phenol has been used in proximity-dependent labeling for protein identification. |
| Biochem/physiol Actions: |
Biotinyl tyramide is a reagent used for tyramide signal amplification for both immunohistochemistry (IHC) and in situ hybridization protocols and with either chromogenic or fluorescence detection. Preliminary binding of a probe is followed by secondary detection of the probe with an HRP-labeled antibody or streptavidin conjugate. Catalysis by the HRP results in the deposition of multiple biotinyl tyramide molecules in the immediate vicinity of the probe that can then be detected with a labeled streptavidin conjugate. Detection sensitivity can be 100-fold or more sensitive compared to conventional detection procedures. |
| Biochem/physiol Actions: |
Reagent used for tyramide signal amplification |
| RIDADR |
NONH for all modes of transport |
| WGK Germany |
WGK 3 |
| Purity |
≥97% (HPLC) |
| Storage Temp. |
−20°C |
| UNSPSC |
12352200 |
