EN6
SIGMA/SML2689 - ≥98% (HPLC)
Synonym: N-
CAS Number: 1808714-73-9
Empirical Formula (Hill Notation): C19H14F2N4O2
Molecular Weight: 368.34
MDL Number: MFCD31665468
Linear Formula: C19H14F2N4O2
Product Type: Chemical
| assay | ≥98% (HPLC) |
| color | white to beige |
| form | powder |
| SMILES string | FC1=C(NC(C=C)=O)C=C(NC(C2 |
| solubility | DMSO: 2 mg/mL, clear |
| storage temp. | 2-8°C |
| Biochem/physiol Actions: | EN6 is an autophagy activator that simultaneously upregulates lysosomal vacuolar H(+)-ATPase (v-ATPase) activity and induces its decoupling from the Rag GTPases by covalently targeting v-ATPase subunit ATP6V1A Cys277. EN6 blocks mTORC1 lysosomal recruitment & activation (1-4 hr 25 μM EN6 pretreatment prior to 10-min amino acids (AA) stimulation of AA-starived HEK293) and clears cellular TDP-43 aggregates (25 μM; U2OS with inducible TDP-43). When administered in mice in vivo (50 mg/kg ip.), EN6 inhibits mTORC1 signaling and activates autophagy in both skeletal muscle and heart tissue. Unlike bafilomycin A1 (BafA1), EN6 activates, but not inhibits v-ATPase catalytic activity. |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |