Synonym: (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-4H-imidazol-4-one; (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4H)-one; DFHBI 1T
CAS Number: 1539318-36-9
Empirical Formula (Hill Notation): C13H9F5N2O2
Molecular Weight: 320.21
MDL Number: MFCD31628972
Linear Formula: C13H9F5N2O2
Product Type: Chemical
| assay |
≥98% (HPLC) |
| color |
white to beige |
| form |
powder |
| solubility |
DMSO: 2 mg/mL, clear |
| storage condition |
protect from light |
| storage temp. |
2-8°C |
| Biochem/physiol Actions: |
DFHBI-IT is a membrane-permeable, non-cytotoxic, RNA aptamers-activated fluorescence probe widely employed in varous detection systems utilizing compatible RNA tags such as Spinach, Spinach2, iSpinach, and Broccoli. Upon binding Spinach2, DFHBI-1T offers brighter fluorescence than DFHBI due to increased extinction coefficient & quantum yield (by 1.4- and 1.3-fold, respectively). DFHBI-1T is also better suited for devices optimized for GFP or fluorescein detection/imaging due to its red shift in excitation & emission wavelengths (Spinach2-comlex Ex/Em = 482/505 nm with DFHBI-1T vs 447/501 nm with DFHBI). |
| WGK Germany |
WGK 1 |
| Flash Point(F) |
Not applicable |
| Flash Point(C) |
Not applicable |
| Purity |
≥98% (HPLC) |
| Storage Temp. |
2-8°C |
| UNSPSC |
12352200 |
