Synonym: Mca-Tyr-Val-Ala-Asp-Ala-Pro-Lys(Dnp), TFA salt; Mca-Tyr-Val-Ala-Asp-Ala-Pro-Lys(Dnp)-OH, TFA salt; Mca-YVADAPK(Dnp), TFA salt (Mca = 7-Methoxycoumarin-4-yl; Dnp = 2,4-dinitrophenyl)
Empirical Formula (Hill Notation): C53H64N10O19 · xC2HF3O2
Molecular Weight: 1145.13 (free base basis)
Linear Formula: C53H64N10O19 · xC2HF3O2
Product Type: Chemical
assay |
≥97% (HPLC) |
color |
white to dark beige |
form |
film or powder |
Quality Level |
100 ![External link](https://krackeler.com/images/sigma/ext-link.gif) |
storage temp. |
−20°C |
Biochem/physiol Actions: |
Mca-YVADAPK(Dnp)-OH is a widely used fluorescent substrate for caspase-1 (ICE cleaves between D & A) and angiotensin-converting enzyme 2 (ACE2 cleaves between P & K). Proteolytic cleavage separates 7-methoxycoumarin-4-acetyl (Mca) from the fluorescence-quenching group 2,4-dinitrophenyl (Dnp), resulting in increased Mca fluorescence (Ex: 320-328 nm, Em: 405-420 nm). |
WGK Germany |
WGK 3 |
Flash Point(F) |
Not applicable |
Flash Point(C) |
Not applicable |
Purity |
≥97% (HPLC) |
Storage Temp. |
−20°C |
UNSPSC |
12352200 |