ppTG21 trifluoroacetate salt
SIGMA/SML3052 - ≥95% (HPLC)
Synonym: GLFHALLHLLHSLWHLLLHA-
Empirical Formula (Hill Notation): C115H173N31O22 · xC2HF3O2
Molecular Weight: 2341.80 (free base basis)
Linear Formula: C115H173N31O22 · xC2HF3O2
Product Type: Chemical
assay | ≥95% (HPLC) |
color | white to beige |
form | film or powder |
Quality Level | 100 ![]() |
storage temp. | −20°C |
Biochem/physiol Actions: | ppTG21 is an endosomolytic agent (pI 7.7, charge +1.3 @ pH 7.4) that facilitates cell type-specific gene editing by enabling endosome escape of Cas9 ribonucleoprotein (RNP) complex following its uptake via receptor-mediated endocytosis. Following liver-specific asialoglycoprotein receptor (ASGPr)-mediated delivery of ASGPr ligand (ASGPrL)-harboring & SV40 nuclear localization sequence (NLS)-bearing S. pyogenes Cas9 (Cas9-2lig-1NLS) RNP with EMX1-targeting sgRNA, target gene editing occurs only upon coincubation of the RNP in the presence of 30 molar equiv of ppTG21 (ave 4.8% indels in HepG2 cells vs. 0.2% indels without ppTG21). |
Purity | ≥95% (HPLC) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |