mirPremier® microRNA Isolation Kit
SIGMA/SNC10 - 1 sufficient for 10 preparations
Synonym: microRNA Isolation Kit; microRNA Isolation Kit
Product Type: Chemical
miRNA quality comparison between Sigma mirPremier microRNA Isolation Kit (A) and the competition (B).
Each sample was purified from 40 mg mouse liver tissue and analyzed with 1% of the total recovery by Agilent Bioanalyzer using an RNA Nano Kit. The two peaks on the right (B) are contaminating rRNA.
Each sample was purified from 40 mg mouse liver tissue and analyzed with 1% of the total recovery by Agilent Bioanalyzer using an RNA Nano Kit. The two peaks on the right (B) are contaminating rRNA.
| Quality Level | 200  |
| technique(s) | DNA extraction: suitable |
| usage | sufficient for 10 preparations |
| Application: | mirPremier® microRNA Isolation Kit has been used to:• prepare small RNA • enrich small RNAfractions from the human astrocytes for small RNA sequencing and bioinformatics analysis • isolate small RNA from Vigna mungo tissues • extract miRNAs to detect the expression of miRNA-16 |
| Features and Benefits: | • Developed to enhance the efficiency of isolating microRNA and other small RNA molecules directly from a wide range of biological samples. • Facilitates rapid and effective extraction and concentration of miRNA in 30 minutes for downstream applications. • Capable of extracting miRNA with high purity with no detectable large RNA. • Hazardous organic extractions are not involved. |
| General description: | Click here to view the kit protocol |
| General description: | Sigma′s mirPremier microRNA Isolation Kit provides a rapid and efficient method for purifying and enriching miRNAs and other small RNAs from diverse biological sources, including mammalian cell cultures, animal tissues, plant tissues, and microbial cultures, without using hazardous organic extractions. microRNAs (miRNAs) are a class of small RNA molecules, about 21 nucleotides (nt) in length, that regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. In addition, the kit also can be used for isolating total RNA if messenger RNA or other large RNAs are of interest. . |
| Legal Information: | mirPremier is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H302 - H315 - H319 - H335 |
| Precautionary statements | P261 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 22-36/37/38 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| UNSPSC | 41105501 |
| Components | Binding Solution 2; Lysis Solution |