Pepsin from porcine gastric mucosa
SIGMA/SRE0001 - Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥3200 units/mg protein
Synonym: Pepsin A; Pepsin from hog stomach
CAS Number: 9001-75-6
EC Number: 232-629-3
MDL Number: MFCD00081840
Product Type: Chemical
application(s) | diagnostic assay manufacturing |
color | white to off-white |
form | lyophilized powder |
impurities | salt, essentially free |
mol wt | 35 kDa |
Quality Level | 400 |
shipped in | wet ice |
specific activity | ≥3200 units/mg protein |
storage temp. | −20°C |
UniProt accession no. | P00791 |
Analysis Note: | Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5. |
Analysis Note: | Protein determined by E |
Application: | Pepsin cleavage can be used to produce F(ab′)2 fragments of antibodies. pepsin at www.sigma-aldrich.com/enz |
Biochem/physiol Actions: | Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin |
Other Notes: | View more information on pepsin at www.sigma-aldrich.com/enz |
Packaging: | 5, 10, 100, 250 g in glass bottle |
Unit Definition: | One unit will produce a ΔA280 of 0.001 per min at pH2.0 at 37° C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16mL. Light path = 1cm.) |
Symbol | GHS07,GHS08 |
Signal word | Danger |
Hazard statements | H315 - H319 - H334 - H335 |
Precautionary statements | P302 + P352 - P305 + P351 + P338 |
Hazard Codes | Xn |
Risk Statements | 36/37/38-42 |
Safety Statements | 22-24-26-36/37 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
activity | specific activity: ≥3200 units/mg protein |
Storage Temp. | −20°C |
Enzyme Commission (EC) Number | 3.4.23.1 ( BRENDA | IUBMB ) |
UNSPSC | 12352204 |