Pepsin from porcine gastric mucosa
SIGMA/SRE0001 - Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥3200 units/mg protein
Synonym: Pepsin A; Pepsin from hog stomach
CAS Number: 9001-75-6
EC Number: 232-629-3
MDL Number: MFCD00081840
Product Type: Chemical
Pepsin, a member of the Peptidase A1 family . It exhibits preferential cleavage for hydrophobic, preferably aromatic.
Pepsin is commonly used in the preparation of F(ab)2 fragments from antibodies. In some assays it is preferable to use only the antigen binding (Fab) portion of the antibody.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: SRE0001-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: SRE0001-10G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: SRE0001-5G
| application(s) | diagnostic assay manufacturing |
| color | white to off-white |
| form | lyophilized powder |
| impurities | salt, essentially free |
| mol wt | 35 kDa |
| Quality Level | 400  |
| shipped in | wet ice |
| specific activity | ≥3200 units/mg protein |
| storage temp. | −20°C |
| UniProt accession no. | P00791  |
| Analysis Note: | Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5. |
| Analysis Note: | Protein determined by E |
| Application: | Pepsin cleavage can be used to produce F(ab′)2 fragments of antibodies. pepsin at www.sigma-aldrich.com/enz. |
| Biochem/physiol Actions: | Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin |
| Other Notes: | One unit will produce a ΔA280 of 0.001 per min at pH2.0 at 37° C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16mL. Light path = 1cm.) |
| Other Notes: | View more information on pepsin at www.sigma-aldrich.com/enz. |
| Packaging: | 5, 10, 100, 250 g in glass bottle |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥3200 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.23.1 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |