Proteinase K from Tritirachium album
SIGMA/SRE0005 - Reagents designed and manufactured under current ISO 13485 certification.
Synonym: Endopeptidase K
CAS Number: 39450-01-6
EC Number: 254-457-8
MDL Number: MFCD00132129
Product Type: Chemical
| application(s) | diagnostic assay manufacturing |
| concentration | ≥10 mg/mL protein |
| ≥800 unit/mL | |
| foreign activity | DNAse, RNAse, exonuclease, endonuclease, and nickase, none detected |
| form | buffered aqueous glycerol solution |
| grade | for molecular biology |
| impurities | ≤500 pg/mg DNA (PicoGreen® assay) |
| mol wt | 28.93 kDa |
| Quality Level | 500  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| Application: | Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A. Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria Determination of enzyme localization on membranes Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling. Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research. |
| Biochem/physiol Actions: | Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA. |
| Legal Information: | PicoGreen is a registered trademark of Life Technologies |
| Unit Definition: | One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent). |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.4.21.64 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |