Thymidine Phosphorylase, recombinant from Escherichia coli
SIGMA/SRE0006 - recombinant, expressed in E. coli, Suitable for manufacturing of diagnostic kits and reagents, buffered aqueous solution, ≥500 units/mL
Synonym: Thymidine:orthophosphate deoxy-D-ribosyltransferase
CAS Number: 9030-23-3
MDL Number: MFCD00132426
Product Type: Chemical
application(s) | diagnostic assay manufacturing |
concentration | ≥500 units/mL |
form | buffered aqueous solution |
Quality Level | 400 ![]() |
recombinant | expressed in E. coli |
shipped in | wet ice |
storage temp. | 2-8°C |
Biochem/physiol Actions: | An enzyme that catalyzes the reversible conversion of thymidine to thymine. Thymidine phosphorylase is part of the pyrimidine nucleoside salvage pathway. This pathway allows pyrimidine bases to be recycled for nucleotide biosynthesis, while the pentose 1-phosphates are converted to intermediates of the pentose phosphate shunt and glycolysis. The E. coli thymidine phosphorylase shares 40% sequence homology with the human sequence, which has been found to be identical to the angiogenic agent platelet-derived endothelial growth factor. The purified E. coli enzyme has been shown to stimulate blood vessel growth in chick chorioallantoic membrane assays. |
Packaging: | 5000 units in glass bottle |
Preparation Note: | Cloned from E. coli and produced in overexpressing E. coli |
Unit Definition: | One unit will convert 1.0 μmole each of thymidine and phosphate to thymine and 2-deoxyribose 1-phosphate per min at pH 7.4 at 25°C. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 2 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | 2-8°C |
Enzyme Commission (EC) Number | 2.4.2.4 ![]() ![]() ![]() ![]() ![]() |
UNSPSC | 12352204 |