Thymidine Phosphorylase, recombinant from Escherichia coli
SIGMA/SRE0006 - recombinant, expressed in E. coli, Suitable for manufacturing of diagnostic kits and reagents, buffered aqueous solution, ≥500 units/mL
Synonym: Gliostatins; Gliostatins; PD-ECGF; PD-ECGF; Thymidine:orthophosphate deoxy-D-ribosyltransferase
CAS Number: 9030-23-3
MDL Number: MFCD00132426
Product Type: Chemical
Unit Definition One unit will convert 1.0 μmole each of thymidine and phosphate to thymine and 2-deoxyribose 1-phosphate per min at pH 7.4 at 25°C.
| application(s) | diagnostic assay manufacturing |
| color | colorless to yellow |
| concentration | ≥500 units/mL |
| form | buffered aqueous solution |
| NCBI accession no. | NP_418799.1   |
| Quality Level | 400 |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| solubility | soluble |
| water: soluble | |
| storage temp. | 2-8°C |
| technique(s) | inhibition assay: suitable |
| UniProt accession no. | P07650 |
| Application: | Thymidine phosphorylase has been used: • in a study to evaluate biomarkers for advanced breast cancer patients treated with capecitabine-based first-line chemotherapy. • in a study to investigate implications for the clinical efficacy of nucleoside analogues. |
| Biochem/physiol Actions: | An enzyme that catalyzes the reversible conversion of thymidine to thymine. Thymidine phosphorylase is part of the pyrimidine nucleoside salvage pathway. |
| Biochem/physiol Actions: | An enzyme that catalyzes the reversible conversion of thymidine to thymine. Thymidine phosphorylase is part of the pyrimidine nucleoside salvage pathway. This pathway allows pyrimidine bases to be recycled for nucleotide biosynthesis, while the pentose 1-phosphates are converted to intermediates of the pentose phosphate shunt and glycolysis. The E. coli thymidine phosphorylase shares 40% sequence homology with the human sequence, which has been found to be identical to the angiogenic agent platelet-derived endothelial growth factor. The purified E. coli enzyme has been shown to stimulate blood vessel growth in chick chorioallantoic membrane assays. |
| Biochem/physiol Actions: | Thymidine phosphorylase catalyzes the reversible conversion of thymidine to thymine. Thymidine phosphorylase is part of the thymidine salvage pathway and pyrimidine nucleoside salvage pathway. This pathway allows pyrimidine bases to be recycled for nucleotide biosynthesis, while the pentose 1-phosphates are converted to intermediates of the pentose phosphate shunt and glycolysis. The enzyme inhibits apoptosis and induces angiogenesis thereby promoting tumor growth and metastatic process. Moreover, thymidine phosphorylase inhibits vascular smooth muscle cell proliferation. |
| General description: | Research area: CELL SIGNALING The E. coli thymidine phosphorylase shares 40% sequence homology with the human sequence, which is identical to the angiogenic agent platelet-derived endothelial growth factor. The purified E. coli enzyme has been shown to stimulate blood vessel growth in chick chorioallantoic membrane assays. |
| Other Notes: | One unit will convert 1.0 μmole each of thymidine and phosphate to thymine and 2-deoxyribose 1-phosphate per min at pH 7.4 at 25°C. |
| Packaging: | 5000 units in glass bottle |
| Preparation Note: | Cloned from E. coli and produced in overexpressing E. coli |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 2.4.2.4 ( BRENDA | IUBMB ); 2.4.2.4 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |