Mutanolysin from Streptomyces globisporus ATCCÂ 21553
SIGMA/SRE0007 - Suitable for manufacturing of diagnostic kits and reagents, ≥4000 units/mg protein (biuret)
Synonym: Mutanolysin
CAS Number: 55466-22-3
MDL Number: MFCD00131635
Product Type: Chemical
Gram Positive Bacterial Cell Wall and Gram Negative Bacterial Cell Wall Mutanolysin lyses Listeria and other Gram-positive bacteria.
Structure of Lysing Enzymes - Mutanolysin and Lysozyme
| application(s) | diagnostic assay manufacturing |
| biological source | bacterial ( |
| concentration | ≥40% protein |
| form | lyophilized powder |
| mol wt | 23 kDa |
| Quality Level | 400  |
| shipped in | wet ice |
| specific activity | ≥4000 units/mg protein (biuret) |
| storage temp. | −20°C |
| suitability | suitable for cell lysis |
| Application: | Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA. |
| Biochem/physiol Actions: | Mutanolysin cleaves the β-N-acetylmuramyl-(1→4)-N |
| Biochem/physiol Actions: | Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N |
| General description: | Mutanolysin from Streptomyces globisporus consists of two main lytic enzymes and may be a useful agent for dental caries control |
| Legal Information: | Ficoll is a registered trademark of Cytiva |
| Other Notes: | One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate. |
| Packaging: | 25000, 100000 units in poly bottle |
| Physical form: | Lyophilized powder containing Ficoll® and sodium succinate buffer salts |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥4000 units/mg protein (biuret) |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |