Interferon Alpha 2A human
SIGMA/SRE0013 - recombinant, expressed in HEK 293 cells, ≥98% (SDS-PAGE), lyophilized powder
Synonym: Interferon α 2A human; INFα2A
Product Type: Chemical
Purity
The purity of human, recombinant Interferon Alpha 2A expressed in HEK 293 cells (Cat. No. SRE0013) was assessed on SDS-PAGE followed by InstantBlue™ staining (Cat. No. ISB1L). (Representative result.)
Lanes
1. 0.5 μg/lane reduced
2. 1.25 μg/lane reduced
3. 2 μg/lane reduced
4. 0.5 μg/lane non-reduced
5. 1.25 μg/lane non-reduced
6. 2 μg/lane non-reduced
The purity of human, recombinant Interferon Alpha 2A expressed in HEK 293 cells (Cat. No. SRE0013) was assessed on SDS-PAGE followed by InstantBlue™ staining (Cat. No. ISB1L). (Representative result.)
Lanes
1. 0.5 μg/lane reduced
2. 1.25 μg/lane reduced
3. 2 μg/lane reduced
4. 0.5 μg/lane non-reduced
5. 1.25 μg/lane non-reduced
6. 2 μg/lane non-reduced
Deglycosylation
Human, recombinant Interferon Alpha 2A expressed in HEK 293 cells (Cat. No. SRE0013) was either treated with Neuraminidase (+) or untreated (-) and separated on SDS-PAGE followed by InstantBlue™ staining (Cat. No. ISB1L). (Representative result.)
Lanes
1. HEK 293 Interferon Alpha 2A - Neuraminidase
2. HEK 293 Interferon Alpha 2A + Neuraminidase
Human, recombinant Interferon Alpha 2A expressed in HEK 293 cells (Cat. No. SRE0013) was either treated with Neuraminidase (+) or untreated (-) and separated on SDS-PAGE followed by InstantBlue™ staining (Cat. No. ISB1L). (Representative result.)
Lanes
1. HEK 293 Interferon Alpha 2A - Neuraminidase
2. HEK 293 Interferon Alpha 2A + Neuraminidase
Bioassay
The biological activity of human, recombinant Interferon Alpha 2A expressed in HEK 293 cells (Cat. No. SRE0013) was measured in a cell inhibition assay using Daudi cells. The EC50 is defined as the effective concentration that elicits a 50% inhibition in cell growth in a cell-based bioassay. (Representative result.)
The biological activity of human, recombinant Interferon Alpha 2A expressed in HEK 293 cells (Cat. No. SRE0013) was measured in a cell inhibition assay using Daudi cells. The EC50 is defined as the effective concentration that elicits a 50% inhibition in cell growth in a cell-based bioassay. (Representative result.)
| assay | ≥98% (SDS-PAGE) |
| form | lyophilized powder |
| Quality Level | 200  |
| recombinant | expressed in HEK 293 cells |
| storage temp. | −20°C |
| Application: | Interferon α 2A human has been used as an inhibitor of hepatitis D virus (HDV). |
| Biochem/physiol Actions: | Interferon Alpha 2A human is produced by many cell types in response to viral infection. IFN-α-2A has antiproliferative and antitumor activities, as well as induction of apoptosis and inflammatory response Interferon α 2A might be implicated in the regression of proliferative diabetic retinopathy. Telaprevir and pegylated interferon–α-2a (PEG-IFN–α-2a) serves as a potential therapeutic method for hepatitis C virus (HCV). IFN α 2A along with IFN α 2b and IFN α2c exhibits anti-proliferative effect. |
| General description: | IFN-α-2a belongs to the type I interferon protein family. Interferon α 2A is one among the three variants of IFN-α2. Recombinant human IFN-α-2a is expressed in human 293 cells as a glycosylated monomer of 165 amino acids, with an average molecular weight of 20 kDa (monomer). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 41106305 |